Through established partnerships with Driving Biomedical Project (DBP) laboratories, the Center on Membrane Protein Production and Analysis (COMPPÅ) will develop new methodologies in the Technology Research and Development (TR&D) areas described below. Once these technologies have been sufficiently established, they will be made available to the membrane protein research community at large.

TR&D 1:

Through mutagenesis approaches, both to expression hosts and gene sequences encoding integral membrane proteins, we will optimize protein expression of targets in both prokaryotic and eukaryotic systems.

TR&D 2:

We will use expression screens of homologous protein targets, detergent and amphipol screens, mutagenesis and protein chimeras, to optimize protein samples for structural studies by x-ray crystallography and cryogenic electron microscopy (cryo-EM).

TR&D 3:

By developing new technologies and improving upon established ones, we will establish generic functional assays so that laboratories may more easily study structure/function relationships of any integral membrane protein of interest.

TR&D 4:

We will develop improved methods for analysis of poorly diffracting, small, or radiation damage-susceptible crystals, such that valuable structural information may still be obtained from these less-than-ideal samples.




New York Structural Biology Center

89 Convent Avenue

New York, NY 10027, USA


Phone: 212-939-0660 extn: 362 or 372

twitter: @comppaa

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