Center on Membrane Protein Production and Analysis
A Biomedical Technology Research Resource
At COMPPAA we use two Monolith machines from Nanotemper Technologies for measuring binding interactions between proteins and ligands. Monolith uses MicroScale Thermophoresis (MST) technology to quantify binding events. One partner is typically labeled with a fluorescent dye and becomes an extremely sensitive reporter for all binding interactions. These events, weak or strong, cause a change in MST signal that is easily detected. The results are automatically translated into precise and quantitative Kd values.
At COMPPAA you can use both label-free proteins (if your protein has enough tryptophan residues and your ligand is not fluorescent) or labeled proteins (in case your ligand is fluorescent).